human circrna array v2 (8 × 15 k)  (Arraystar inc)

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: Primer sequences used in the validation of circRNAs.

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques: Biomarker Discovery, Sequencing

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: The top 15 upregulated and 15 downregulated circRNAs in gout patients compared with healthy control subjects.

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques: Control

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: Expression profiles of circRNAs in gout patients compared with healthy controls. (A) Scatter plot demonstrating the heterogeneity of the expression of circRNAs in the gout and HC groups. The values of the X and Y axes represent the averaged normalized signal values of the groups (log2-scaled). The green line represents 1.5-fold changes. The expression of circRNAs above the top green line and below the bottom green line indicates changes by > 2-fold between the two groups. (B) Volcano plots visualizing differentially expressed circRNAs between the two groups. The vertical lines correspond to 1.5-fold up- and downregulated circRNA expression. The horizontal line represents a p -value of 0.05. The red point in the plot represents significantly differentially expressed circRNAs. (C) Clustered heatmap showing the relationships among the expression levels of samples. Expression values are represented by the color scale. The intensity increases from green (relatively low expression) to red (relatively high expression). Each column represents one sample, and each row represents a single circRNA. (D) Genomic region distribution of upregulated circRNAs. (E) Genomic region distribution of downregulated circRNAs. (F) Distribution of significantly dysregulated circRNAs in chromosomes. (G) Relative expression levels of the six circRNAs in five gout patients and three healthy control (HC) subjects. The Y-axis represents the ratio of the relative expression level of circRNAs in the gout group to that of the HC group. Gout: primary gout; HC: healthy controls.

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques: Expressing, Control

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: Prediction and annotation of hsa_circRNA_103657 and hsa_circRNA_000241 ceRNA mechanism. (A) Construction of the circRNA-miRNA-mRNA network: the network of ceRNA includes 2 circRNAs, 10 miRNAs, and 525 mRNAs. (B) Schematic diagram of the gene category of the PI3K-Akt signaling pathway, which is the top enriched term in the KEGG pathway analysis. Image from the Kyoto Encyclopedia of Genes and Genomes (KEGG) resource ( http://www.genome.jp/kegg/ ).

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques:

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: Clinical significance of hsa_circRNA_103657 and hsa_circRNA_000241 in primary gout (A,B,C,D) Correlations between the expression levels of circRNAs (hsa_circRNA_103657 and hsa_circRNA_000241) and laboratory data of gout, analyzed using Spearman’s coefficient. (E, F) Receiver operating characteristic (ROC) curve for the analysis of the diagnostic value of hsa_circRNA_103657 and hsa_circRNA_000241 for primary gout. AUC: area under the curve.

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques: Expressing, Diagnostic Assay

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Primer details for quantitative real-time reverse transcription–polymerase chain reaction of circRNA_000585.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Heatmap of circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens (P1–3) and matched para-cancer specimens (C1–3) from three patients with CCA. Each block represents different circRNA expression levels (red represents high expression; green represents low expression).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Blocking Assay, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Fold change in circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens and matched para-cancer specimens from three patients with CCA. (A) scatter plot (dots above the upper line represent fold-change >1.5, dots below the lower line represent fold-change >–1.5); and (B) volcano plot of circRNA expression (red dots represent fold-change >1.5 or >–1.5).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Vertical scatter plot showing elevated circRNA_000585 expression in cholangiocarcinoma (CCA) tumour tissue versus matched para-cancer tissue from 15 patients with CCA (central horizontal line represents mean, upper and lower horizonal lines represent SD; P = 0.003 between groups).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Association between circRNA_000585 expression and clinicopathological characteristics in 15 patients with cholangiocarcinoma.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer detection

doi:

Figure Lengend Snippet: Candidate CRC-related circRNA identification. A. Differentially expressed circRNAs from the plasma of CRC patients and healthy controls (n=3) were subjected to hierarchical clustering analysis. B. qRT-PCR analysis of RNase R-resistant circRNAs, with GAPDH serving as a negative control. C. No changes in Ct values for these three circRNAs were detected following a 24 h incubation at room temperature, as measured via qRT-PCR. D-F. Correlations between plasma and intratumoral levels of these differentially expressed circRNAs in CRC patients (n=15).

Article Snippet: Resultant labeled cDNAs were then hybridized onto an Arraystar human circRNA Array V2 (8×15 K), after which an Agilent Scanner G2505C was used to scan and analyze these arrays. qRT-PCR Power SYBR Green (Takara, Dalian, China) was used for qRT-PCR analyses of pairs of CRC and non-tumor tissues, with GAPDH used as a control for relative gene expression, which was assessed via the 2 -ΔΔCq method.

Techniques: Clinical Proteomics, Quantitative RT-PCR, Negative Control, Incubation

Journal: American Journal of Translational Research

Article Title: Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer detection

doi:

Figure Lengend Snippet: Validation of candidate CRC-related circulating circRNA profiles in an independent patient cohort. A. Levels of hsa_circ_0001900, hsa_circ_0001178, and hsa_circ_0005927 were assessed in the plasma of 102 CRC patients, 42 healthy controls, and 30 patients with precancerous lesions via qRT-PCR. B. Levels of hsa_circ_0001900, hsa_circ_0001178, and hsa_circ_0005927 were assessed via qRT-PCR in 20 paired plasma samples from CRC patients before and after surgery.

Article Snippet: Resultant labeled cDNAs were then hybridized onto an Arraystar human circRNA Array V2 (8×15 K), after which an Agilent Scanner G2505C was used to scan and analyze these arrays. qRT-PCR Power SYBR Green (Takara, Dalian, China) was used for qRT-PCR analyses of pairs of CRC and non-tumor tissues, with GAPDH used as a control for relative gene expression, which was assessed via the 2 -ΔΔCq method.

Techniques: Biomarker Discovery, Clinical Proteomics, Quantitative RT-PCR

Journal: Chinese Medical Journal

Article Title: Identification of Circular RNAs as a Novel Biomarker for Ovarian Endometriosis

doi: 10.4103/0366-6999.226070

Figure Lengend Snippet: qRT-PCR validation of the four selected circRNAs. (a) The results of microarray and qPCR are shown as blue and red columns. Data that coincided with the microarray results and met the statistical cut-off are marked with * P < 0.05 and † P < 0.001. (b) Effect of menstrual cycle on the two identified circRNAs. Data are expressed as fold changes relative to the values for the proliferative phase group of controls. Both the circRNAs show no significant changes between proliferative (red columns) and secretory phases (blue columns). qRT-PCR: Quantitative real-time polymerase chain reaction; qPCR: Quantitative polymerase chain reaction; circRNAs: Circular RNAs.

Article Snippet: The Arraystar Human circRNA Array 8 × 15 K V2 Microarray (Arraystar, Rockville, MD, USA) contains 15,000 probes for 13,617 human circRNAs, all of which have been confirmed by Jeck et al .,[ ] Salzman et al .,[ ] Memczak et al .,[ ] Zhang et al .,[ ] Zhang et al .,[ ] Guo et al .,[ ] and You et al .[ ] Total RNA was first digested with Rnase R (Epicentre, Madison, WI, USA) to remove linear RNAs and enrich for circRNAs.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Microarray, Real-time Polymerase Chain Reaction